Interference by gelatin-based plasma substitutes in capillary zone electrophoresis.

could not determine the CYP2B6 genotype of this individual because the PCR-RFLP assays failed to distinguish the two genotypes. An individual classified as CYP2B6*1/*7 or *5/*6 has also been reported by other authors (4). It is necessary to distinguish CYP2B6*1/*7 and *5/*6 because CYP2B6*5, *6, and *7 have been associated with changes in the production and function of CYP2B6, respectively (2, 4). In haplotype determination , a cloning method is usually performed, but this is time-consuming. We therefore developed a method for rapid haplotype determination based on PCR to distinguish CYP2B6*1/*7 and *5/*6.